The Frenchman made his first appearance of the season on Saturday in the 3-0 win over Eibar but will now sit out another game with Clement Lenglet set to partner Gerard Pique.
Sergi Roberto, who was forced off with a knee injury against Eibar, is also absent from the trip, but Barca otherwise have a fully fit squad for the game in the Czech Republic.
“In the final part of the session on Tuesday at the Ciutat Esportiva Samuel Umtiti picked up a bruised knee and so the French central defender misses the trip to take on Slavia Prague.
“Sergio Roberto also misses out as he is still recovering himself from a knee injury picked up in the win at the weekend against Eibar in La Liga.”
Source | FC Barcelona
Barcelona head into Wednesday’s match level with Borussia Dortmund on four points at the top of Group F. Dortmund play Inter Milan at the San Siro in the group’s other game.