The midfielder picked up a yellow card during the match which means he will be banned for the second leg. Arturo Vidal is also suspended after picking up a late red card.
Barcelona are already without Luis Suarez, Ousmane Dembele, Jordi Alba and Sergi Roberto due to injury, meaning Quique Setien is short of options for the second leg.
Here’s what Busquets makes of it all:
Barca midfielder Busquets to Movistar: We don’t have a deep squad because, unfortunately, that’s how it was planned.— Samuel Marsden (@samuelmarsden) February 25, 2020
Barcelona also have injury concerns over Gerard Pique ahead of Sunday’s Clasico at the Santiago Bernabeu. The centre-back hurt his ankle late on in the clash at Napoli and looks to be a doubt for the trip to Madrid.